Table 2.

Detail of primers used for the expression profiling of different wheat cystatin families

S no.	Primer code	Sequence (5′→3′)	No. of bases	Tm (°C)	% GC
1	WC2F	CCG TCT CCG AGC ACA AGA	18	55	61
2	WC2R	ATC CTT GCA CAG CTG GGA	18	55	55
3	WC3F	CCA TCG TCG TGC CGT TTA CTC	21	55	57
4	WC3R	TAC ACG AGA ACA CGC TAA ACC A	22	55	45
5	WCMDF	CAT AAT ATC CAG GTA GAG ACA GGT	24	55	45
6	WCMDR	ATT CAC TGG CTG CTA GAT TCG TCA	24	55	46
7	Wac F	AGT GTC TGG ATC GGT GGC TCT ATT	24	55	50
8	Wac R	TCC CCT TCA CCG ACT CTT CAA AA	23	55	48