Figure 1.

The phenotypes of sne-t2 and sne-t3 T-DNA mutants. (A) Schematic diagram of the sne-t2 T-DNA insertion at position −1 bp and of sne-t3 at position +435 bp with respect to the translation start site. (B) Final plant height (upper) and fertility (lower) of indicated genotypes. Letters indicate statistically different classes as determine by t-test. Bars represent standard error. (C) sne mutants show increase in ABA sensitivity. Seeds of wild-type Ws, sne-t2 and sne-t3 were after-ripened for 2 weeks then sown on MS-agar containing indicated concentrations of ABA as described by Steber et al.¹¹ Germination was scored based on radical emergence after incubating 3 days at 4°C followed by 14 days at 22°C. (D) Mutations in SNE cause no significant effect on DELLA RGA, GAI and RGL2 protein accumulation. Total protein was extracted from leaves of 12-d-old seedlings (Top) or flower buds (FB, bottom) and detected as described in Ariizumi et al.⁴