Skip to main content
. 2011 May 1;8(3):365–371. doi: 10.4161/rna.8.3.14804

Figure 1.

Figure 1

CUGBP1 associates to a U-rich region of the p27 5′-UTR. (A) Sequence of the U-rich region of the human p27 5′-UTR. (B) Diagram of the human p27 5′-UTR with the U-rich region shown in black. Truncations and deletions used for RNA-binding assays are shown. (C) Binding of CUGBP1 to the p27 5′-UTR. Extracts prepared from MCF7 were used for biotin-labeled RNA pulldown assays. Biotin-labeled probes included a control RNA derived from a plasmid vector, the full-length p27 5′-UTR, and the deletions/truncations shown in (B). RNA-bound CUGBP1 was eluted and assayed by western blotting. For the input lane total MCF7 cell extract was used.