Table 1.

Primer sequences

Primer	Sequence	Template	Comments
5′CUGBP-PC	AGG ATC CAA TGA ACG GCA CCC TGG A	GFP-CUGBP1 plasmid	primers for subcloning hCUGBP1 cDNA into pCDNA3.1-HisB
3′CUGBP-PC	ACT CGA GTC AGT AGG GCT TGC TGT CA
5′CUGBP-PR	AGC TCG AGA ATG AAC GGC ACC CTG GA	pCDNA3.1-HisB-CUGBP1	primers for subcloning hCUGBP1 cDNA into pRSETB
3′CUGBP-PR	AGA AGC TTT CAG TAG GGC TTG CTG C
5′p27UTR	GGA TCC TAA TAC GAC TCA CTA TAG GCT TCT TCG TCA GCC TCC CTT	pTKLL472	primers for p27 5′UTR DNA amplification
3′p27UTR	CTT TCT CCC GGG CCG TGG CTC GTC GGG G
5′p27UTR226	GGA TCC TAA TAC GAC TCA CTA TAG GCC TCT CCG CCC TCC CGC TCG C	pTKLL472	primers for p27 5′UTR 226–472 truncation DNA amplification
3′p27UTR	CTT TCT CCC GGG CCG TGG CTC GTC GGG G
5′p27UTR	GGA TCC TAA TAC GAC TCA CTA TAG GCT TCT TCG TCA GCC TCC CTT	pTKLL472	primers for p27 5′UTR 1–219 truncation DNA amplification
3′p27UTR219	CTT TCT CCC GGG CCG TGG CTC GTC GGG G
5′p27UTR	GGA TCC TAA TAC GAC TCA CTA TAG GCT TCT TCG TCA GCC TCC CTT	pTKLL472-ΔU	primers for p27 5′UTR U-rich region deletion DNA amplification
3′p27UTR	CTT TCT CCC GGG CCG TGG CTC GTC GGG G
5′-472-Luc-PolA	GGA TCC TAA TAC GAC TCA CTA TAG GCT TCT TCG TCA GCC TCC CTT	pTKLL472	primers for p27 5′UTR-Luciferase-poly A DNA amplifcation
3′-472-Luc-PolA	TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT AGC TAA GAA TTT CGT CAT CG
5′-rev472-Luc-PolA	TAA TAC GAC TC A CT A TAG GAA AGA GGG CCC AGA CGT	pTKLL472R	primers for p27 rev5′UTR-Luciferase-poly A DNA amplifcation
3′-rev472-Luc-PolA	TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT AGC TAA GAA TTT CGT CAT CG
5′-ctrl-Luc-PolA	TAA TAC GAC TC A CTA TAG GG	pCDNA3.1-Luc	primers for Luciferase-poly A DNA amplifcation
3′-ctrl-Luc-PolA	TTT TTT TTT TTT TTT TTT TTT TTT TTT TTT AGC TAA GAA TTT CGT CAT CG