Figure 4.

The effect of fluvastatin and Mito-CP₁₁ on the extent of cell death in MCF-7 and MCF-10A cells. (A) MCF-7 and MCF-10A cells were treated with Mito-CP₁₁ (0.5 µM) with or without fluvastatin (1 µM) and mevalonate (20 µM) for 48 h, and dead cells were monitored by staining with SYTOX Green. To measure the total cell number, cells were treated with digitonin (120 µM) when staining with SYTOX Green. Fluorescence intensity from cells grown in 96-well plate was measured using a plate reader (excitation wavelength, 485 nm and the emission wavelength 535 nm). (B) Fluorescence microscopy pictures were obtained using FITC filters under the same experimental cell culture conditions as in (A). Asterisks above a column indicate a statistical comparison between the indicated treatment and the control (*p < 0.05).