Figure 5.

Uptake of Mito-CP₁₁ into mitochondria. MCF-7 cells were treated with CP (5 µM) in the presence of Me-TPP⁺ and Mito-CP₁₁ (5 µM) for 48 h. After treatment, cells were fractionated to isolate mitochondria and cytosol. CP and Mito-CP₁₁ distributions in media, cytosol, mitochondria and cell lysate were determined using EPR. The EPR signals from different samples were normalized to the same concentration of protein.