Figure 1.

Simultaneous establishment of tumor growth in the lung, liver and kidney with B16-F1 cells. B16-F1 melanoma cells in serum-free medium were tail vein injected following either the hydrodynamic (7.5% body weight in volume, 5 sec injection time) (A–G) or conventional (H–N) method at a dose 1 × 10⁶ cells/mouse. Mice were sacrificed 15 d after the injection, and the lungs, livers and kidneys were harvested and fixed immediately in 10% neutral buffered formalin. Pictures were taken using a Canon G9 digital camera. Five micrometer tissue sections were made for each organ and H&E staining was performed. Arrows point to the tumor sites.