Figure 3.
Simultaneous establishment of tumor growth in the lung, liver and kidney with Renca cells. Renca cells suspended in serum free medium were tail vein injected following the hydrodynamic (A–G) (9% body weight in volume, 5 sec) or conventional (H–N) (100 µl, 5 sec) procedure at a dose of 5 × 105 cells/mouse. Mice were sacrificed 20 d after tumor injection, and the lungs, livers and kidneys were collected and immediately fixed in 10% neutral buffered formalin. Pictures were taken using a Canon G9 digital camera. Arrows point the tumor sites.