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. 2011 Oct 15;12(8):750–755. doi: 10.4161/cbt.12.8.16867

Figure 1.

Figure 1

Structure of TYMS 5′-UTR tandem repeats-luciferase reporter constructs having Kozak sequences from firefly luciferase (LK) and human TYMS (HK) are shown. Leucine (CTT) was inserted by site mutagenesis between methionine (ATG) and glutamine (GAA) to generate constructs with native TYMS Kozak sequence GCCATGC in place of the firefly Kozak GCCATGG.