Table 1.
Binding and inhibitory activities of scFv b12 variants measured in ELISA and a TZM-bl cell line-based pseudovirus assay, respectively
| scFv b12 variants | EC50 (nM) | IC50 in TZM-bl Pseudovirus Assay (nM) | ||||||
| gp120Bal | JRFL (B) | Bal (B) | ADA (B) | JRCSF (B) | CH1 81.12 (CRF07-BC′) | PCNE1 (B′) | GXC 44 (C) | |
| b12 gerHV-gerD(J)-gerVL | >1,650 | >1,650 | >1,650 | >1,650 | >1,650 | >1,650 | >1,650 | >1,650 |
| b12 matHV-gerD(J)-gerVL | 12.7 | >1,650 | >1,650 | >1,650 | >1,650 | >1,650 | >1,650 | >1,650 |
| b12 gerHV-matD(J)-gerVL | 1,008.7 | >1,650 | >1,650 | >1,650 | >1,650 | >1,650 | >1,650 | >1,650 |
| b12 gerHV-gerD(J)-matVL | 835.7 | >1,650 | >1,650 | >1,650 | >1,650 | >1,650 | >1,650 | >1,650 |
| b12 matHV-matD(J)-gerVL | 2.3 | >1,650 | >1,650 | >1,650 | >1,650 | >1,650 | >1,650 | >1,650 |
| b12 gerHV-matD(J)-matVL | 24.1 | 1,364.7 | >1,650 | >1,650 | >1,650 | >1,650 | >1,650 | >1,650 |
| b12 matHV-gerD(J)-matVL | 2.4 | 15.9 | 55.6 | 1,067.2 | >1,650 | 855.7 | >1,650 | >1,650 |
| b12 matHV-matD(J)-matVL | 0.4 | 8.4 | 13.0 | 170.9 | 77.5 | 281.8 | >1,650 | >1,650 |
EC50s and IC50s were determined using GraphPad prism software. ger, germline; mat, mature.