To investigate the in vivo function of SATB2 in osteogenic differentiation, qRT-PCR analysis was performed to detect the expression levels of SATB2, Osx, Runx2, BSP, COLI and OC in the newly formed bone tissues. (a)Gene expression in pBABE-Satb2 group and pBABE-hygro group1 week after implantation; (b) Gene expression in pBABE-Satb2 group and pBABE-hygro group3 week after implantation; (c)Gene expression in RCASBP(A) group and RCAS-Satb2 group 1 week after operation; (d) Gene expression in RCASBP(A) group and RCAS-Satb2 group 3 week after operation. Data were expressed as mean±SEM (n=3). *p<0.05.