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. 2011 Sep 15;12(6):503–509. doi: 10.4161/cbt.12.6.15976

Figure 1.

Figure 1

ARF interacts with HSP70. (A) Two Dimensional Differential In Gel Electrophoresis (2D-DIGE) of mitochondrial ARF-interacting proteins in mitochondria purified from U2OS-ARF cells. Immunoprecipitates of ARF-induced cells were labeled with CyDye; the circled spots indicate ARF-binding proteins that were excised and sequenced by mass spectrometry. Several spots identified as HSP70 are shown. (B) Co-immunoprecipitation of HSP70 with antisera to ARF (negative control, lane 3) in U2OS-ARF cells treated with doxycycline (Doxy) for 24 h. Normal rabbit IgG is the negative control. IP: immunoprecipitation. On the right, the level of HSP70 and ARF in whole cell lysate from the same samples is depicted. (C) Co-immunoprecipitation of HSP70 with endogenous ARF (negative control IgG in lane 2) in mouse embryo fibroblasts (MEFs) from the p53-/- mouse. (D) Co-immunoprecipitation of HSP70 with endogenous ARF (negative control IgG in lane 2) in a T-cell lymphoma line isolated from the p53-/- mouse (T313). (E) Co-immunoprecipitation of endogenous ARF with HSP70 in p53-/- MEFs. (F) Co-immunoprecipitation of HSP70 with ARF antisera in mitochondrial (Mito) and cytosolic (Cyto) lysates isolated from U2OS-ARF cells treated with doxycycline (doxy) for 24 h.