Figure 2.

Requirement for membrane localization of Akt for resistance to tamoxifen. MTT was utilized to monitor the requirement of membrane localization of Akt for resistance to tamoxifen (4HT). MCF7/ΔAkt-1:ER*(Myr⁺) and MCF7/ΔAkt-1:ER*(Myr⁻) were plated in different concentrations of doxorubicin in either the presence and absence of 500 nM 4HT and proliferation was estimated by MTT analysis. The dotted arrow represents where 50% inhibition of growth intercepts with the X axis and is used to estimate the IC₅₀. The statistical significance in (B) was determined by the unpaired t-test (***p < 0.0001).