Figure 4.

Elevated Akt expression in 4HT-selected cells. MCF7/ΔAkt-1:ER*(Myr⁺) cells were cultured in the indicated conditions (+/- 4HT, in the presence and absence of phenol red containing medium) for 4 weeks. Cells were then collected after trypsinization and plated in phenol red free RPMI 1640 containing 10% FBS. After allowing the cells to adhere for 24 h, the treated with 1,000 nM 4HT for the indicated time periods. Protein lysates were prepared and subjected to protein gel blot analysis.