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. 2011 Sep 1;10(17):3003–3015. doi: 10.4161/cc.10.17.17119

Figure 8.

Figure 8

Effects of Akt activation on colony formation in the presence and absence of radiation, doxorubicin and 4HT. MCF7/ΔAkt-1:ER*(Myr+) (4HT+Dox) cells were culture for 4 weeks in RPMI 10% FBS, labeled (ND = no drugs), RPMI + 10% FBS + 1,000 nM 4HT, labeled (4HT) or RPMI + 10% FBS + 1,000 nM 4HT + 100 nM doxorubicin, labeled (4+D). Colony counts were normalized to the number of colonies observed in untreated cells (n = 3). (A) Colony formation of MCF-7 and MCF7/ΔAkt-1:ER*(Myr+)(4HT+Dox) in RPMI + 10% FBS. (B) Colony formation of MCF-7 and MCF7/ΔAkt-1:ER*(Myr+) (4HT+Dox) in RPMI + 10% FBS + 1,000 nM 4HT. (C) Colony formation of MCF-7 and MCF7/ΔAkt-1:ER*(Myr+)(4HT+Dox) in RPMI + 10% FBS + 100 nM doxorubicin (Dox). (D) Colony formation of MCF-7 and MCF7/ΔAkt-1:ER*(Myr+)(4HT+Dox) in RPMI + 10% FBS + 100 nM 4HT + 100 nM doxorubicin (4HT+D).

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