Multiplex RT-PCR analysis of ITGA9 expression with different primer pairs. (A) Scheme of the primers used in the study. (B) Representative gel from multiplex RT-PCR. DNA fragments amplified with two different primers pairs are shown. GAPDH expression was used as an internal standard. (C) ITGA9 expression levels normalized to that of GAPDH (TotalLab Programme). The graph shows the mean expression levels from triplicate experiments (± SD) (OriginPro 8.1). 1 and 2, normal breast tissue samples; 109, 110, 125 and 126, breast tumors; C and T, control and tumor breast tissue (match pair for each patient), respectively.