Methylation of ITGA9 CpG-island in primary human breast tumors. (A) ITGA9 expression in breast tumors; a representative gel from multiplex RT-PCR. (B) Methyl-specific PCR on ITGA9 CpG-island. (C) Bisulfite sequencing. 301–328, breast tumors; C and T, control and tumor breast tissue (match pair for each patient); +, positive PCR control; −, negative PCR control; M, DNA marker; Meth and Unmeth, primers for methylated or un-methylated DNA sequence, respectively.