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. 2011 Sep 15;10(18):3111–3118. doi: 10.4161/cc.10.18.17300

Figure 2.

Figure 2

Notch3 is induced by ectopic ΔNp63α and other quiescence-inducing stimuli. (A) ΔNp63α specifically induces Notch3 mRNA in HC11 cells. Infection with adenovirus expressing ΔNp63α or GFP for 48 h significantly induced Notch3 mRNA as assessed by qPCR (p < 0.05, Student t-test). (B) ΔNp63α induces Notch3 mRNA in MCF7 breast cancer cells. (C) ΔNp63α activates signaling of the Notch3 receptor. In addition to elevating total Notch3, ΔNp63α overexpression also increases the amount of cleaved Notch3 intracellular domain, an indicator of active Notch signaling, as assessed by protein gel blot. (D) ΔNp63α induces Hes1, a canonical Notch signaling target and mediator of cellular quiescence. (E) Notch3 is induced following multiple quiescence-promoting stimuli in addition to ΔNp63α. As assessed by qPCR, Notch3 mRNA is elevated following serum deprivation (SD), low-binding culture (LB) and contact inhibition (CI) (p < 0.05).