Figure 2.

Effect of HDAC inhibitors in combination with VP-16 on cell cycle in H146 cells. (A) Cells were treated with belinostat (BEL) (0.3 µM), VP-16 (4 µM) or combination (BEL + VP-16). Top part shows control untreated cells or cells treated with a single drug for the indicated times. The bottom part shows simultaneously treated cells (left) and sequentially treated cells with belinostat added for 24 h prior to VP-16 (right), as indicated. (B) Same conditions as above using romidepsin (DP) at 1 ng/mL. The numbers in each part represent percent cells in each cell phase of cell cycle. A representative example of three independent experiments for each condition is shown here.