Figure 9.

VAChT-Cre reduces TrkB in pre-ganglionic sympathetic neurons. (A) Thoracic spinal cord frozen sections from 8-month-old ROSA 26 reporter mice expressing Cre under the control of the VAChT promoter were stained with X-gal, which showed intense labeling of cells was evident in the IML and in somatic motor neurons (SMN). (B and C) Thoracic spinal cord frozen sections were stained with Cresyl Violet, and neurons in IML (arrowheads) were microdissected with PALM system (50×). Scale bars, 100 μm. (D) Single-cell RT-PCR and nested PCR analyses of microdissected IML neurons for TrkB (upper panel), Cre (middle panel) and ChAT (lower panel) expression.