Figure 1.

Neutralization of homologous genotype or subtype hepatitis C virus (HCV) pseudoparticles (HCVpp) by serum samples obtained from children with high (upper panel) or normal to low (lower panel) alanine aminotransferase (ALT) levels at the indicated months. Neutralizing antibodies (NtAbs) were detected using HCVpp bearing E1 and E2 glycoproteins of the homologous genotype or subtype as indicated. The percentage of neutralization was calculated by comparison with the mean results obtained with 3 normal anti-HCV negative human serum samples. Neutralization was defined as ≥50% reduction of green fluorescent protein–positive cells; 50% neutralization is indicated by horizontal line. Replicates for neutralization test in serial serum samples collected longitudinally from each patient were not performed because of the limited amount of available serum.