Figure 5.

Accumulation of coexpressed fluorescent proteins (FPs) in nucleocapsid protein-induced inclusions. A, Fluorescence microscopy analysis of HT-1080 cells expressing TagRFP (red), DsRed (orange), or mCherry (pink), along with MARV NP and VP35. Cells were either stained with anti-NC antiserum (green) and DAPI (blue) or DAPI alone (right panels). B, Fluorescence microscopy analysis of HT-1080 cells coexpressing EGFP along with ZEBOV (upper panel) or REBOV (lower panel) NP and VP35 proteins. Cells were stained with DAPI (blue) and an antibody detecting the nucleocapsid proteins of both virus species (red).