Figure 6.

The presence of overexpressed DRBP76 disrupts VP35-NP interaction. A, Graph depicting polymerase activity from the EBOV minigenome co-transfected with empty plasmid, DRBP76-Flag, GFP-Flag or no VP35 (negative control). Each bar represents duplicate samples. B, Fifty percent of the total material from each well in (A) was immunoprecipitated with an anti-VP35 antibody and measured by western blot with antibodies to VP35 or NP. C, Western blot of the total cell extract from the same samples as (B).