Figure 4. ROS production is increased in Lnk KO macrophages.

(A) Zymosan internalization of bone marrow-derived macrophages from WT and Lnk KO mice. Cells were fed with FITC-labeled zymosan particles for 30 min. Fluorescence of internalized zymosan was assessed by flow cytometry in comparison with unlabeled macrophages. (B) Bone marrow-derived macrophages from Lnk KO and WT mice were plated overnight in factor-free culture media or media containing M-CSF. After stimulation with zymosan, ROS production was measured by luminol-enhanced chemiluminescence [relative light units (RLU)] at the indicated time-points. Diagram shows results of unprimed and M-CSF-primed macrophages, with or without zymosan stimulation.