Table 2.

Comparison of fold change between oligonucleotide arrays and RT-PCR

	Avg Diff (array)		Fold change
Gene	hs-wt	hs-lab	Array	RT-PCR
lab	41	1078	26.3	55.7
swa	20	406	20.3	18.4
UbcD4	44	423	9.6	6.5
twe	20	132	6.6	4.9
cycB	243	1344	5.5	4.6
Uch	61	312	5.1	12.1
sqd	373	370	1.0	1.1
scrt	225	79	-2.9	-3.7
Pepck	610	171	-3.6	-4.6

RT-PCR was performed on cDNA derived from heat-shocked wild-type embryos and heat-shocked hs-lab embryos. Fold changes determined by RT-PCR are represented as the mean values of eight independent replicates, derived from two different cDNA preparations. Avg Diff, absolute average difference value (see Materials and methods).