Figure 1.

Time-dependent infiltration of neutrophils and phosphorylation of p38 in the skin. A dose of 50 μg/mL LPS in a 50 μL injection volume induced neutrophil-mediated skin inflammation as measured by MPO activity increased monotonically over time, beginning at 6-hours post-injection and reaching a maximum level by 24-hours when compared to the two-hour time point (A; *P < 0.05; *P < 0.005; ***P < 0.001). Phosphorylated p38 MAPK was observable in neutrophils and endothelial cells beginning at 8-hours post-injection and continued for 48-hours (B; *P < 0.05; **P < 0.01; ***P < 0.001 compared to two-hour time point). Values are reported as the mean ± SD; n = 10.

Abbreviations: LPS, lipopolysaccharide; MAPK, mitogen-activated protein kinase; MPO, myeloperoxidase; pp38, phosphorylated p38; SD, standard deviation.