Figure 4.

2-DG inhibits PMA-induced nuclear translocation of the p65 subunit of NF-κB. A) HBMEC were serum-starved for 30 min in the presence of either vehicle or 100 mM 2-DG. Cells were then incubated for the indicated time with 1 μM PMA. Nuclear extracts were isolated, electrophoresed via SDS-PAGE and immunodetection of the p65 subunit of NF-κB protein was performed as described in the Methods section. B) Quantification was performed by scanning densitometry of the autoradiograms. Data were expressed as x-fold induction over basal untreated cells of the vehicle pretreated cells (open circles) and 2-DG pre-treated cells (closed circles).

Abbreviations: HBMEC, mitovasuila endothelial cells; PAGE, pohyacrylanide electrophoresis. PMA, phorbol 12-myristate 13-acetate; 2-DG-glucose.