Table 1.
Summary of trans-spliced and permuted tRNAs
| 5' half of tRNA | 3' half of tRNA | |||||||
|---|---|---|---|---|---|---|---|---|
| Organism | tRNA isotype | Anticodon | 5' Start | Size (bp) Pre/Mature | 5' Start | Size (bp) Pre/Mature | Ligation site | Intron position |
| *Aeropyrum pernix | Asp | GUC | 372080 (+) | ~67/40 | 372241 (+) | ~58/35 | 37/38 | |
| *Thermosphaera aggregans | Asp | GUC | 41001 (+) | ~53/40 | 41160 (-) | ~53/35 | 37/38 | |
| *Staphylothermus hellenicus | Lys | CUU | 1095046 (+) | ~48/31 | 1090266 (+) | ~65/43 | 30/31 | |
| *Staphylothermus marinus | Lys | CUU | 1279876 (-) | ~48/31 | 1284654 (-) | ~65/43 | 30/31 | |
| Nanoarchaeum equitans | Lys | CUU | 308963 (+) | ~44/32 | 380370 (-) | ~60/43 | 30/31 | |
| Gln | UUG | 419223 (-) | ~49/33 | 356559 (+) | ~61/40 | 32/33 | ||
| Glu | CUC | 331758 (-) | ~56/40 | 487064 (-) | ~56/35 | 37/38 | ||
| Glu | UUC | 436673 (-) | ~56/40 | 487064 (-) | ~5635 | 37/38 | ||
| His | GUG | 221794 (+) | ~49/37 | 409246 (+) | ~53/36 | 37/38 | ||
| iMet | CAU | 35249 (+) | ~53/39 | 3254 (+) | ~5236 | 37/38 | ||
| *Thermofilum pendens | iMet | CAU | 539254 (-) | ~89/75 | 539278 (-) | ~43/17 | 59/60 | 37/38 |
| Tyr | GUA | 892743 (+) | ~128/117 | 892725 (+) | ~34/17 | 59/60 | 37/38 | |
| Cyanidioschyzon merolae | Ala | UGC | chr6:137501 (-) | ~63/58 | chr6:137523 (-) | ~32/14 | 59/60 | |
| Arg | CCU | chr10:636110 (+) | ~88/85 | chr10:636073 (+) | ~47/14 | 59/60 | 14/15 | |
| Lys | UUU | chr16:809263 (-) | ~60/57 | chr16:809315 (-) | ~70/14 | 59/60 | ||
The novel trans-spliced and permuted tRNAs identified in this study are marked by asterisks. The previously identified split tRNAs in N. equitans [4,5] and permuted tRNAs in C. merolae [9] are listed for comparison. The 5' start value represents the coordinates of the 5' start site of the mature tRNA. Pre, pre-tRNA; Mature, mature tRNA.