Table 1.
Primer information for real-time PCR
| Gene accession number | Sequence (5'-3') | Size, bp | Annealing temperature, °C | |
|---|---|---|---|---|
|
tlr1 (rat) [NM_001172120] |
Forward | CAGCAGCCTCAAGCATGTCTA | 82 | 60 |
| Reverse | CAGCCCTAAGACAACAATACAATAGAAGA | |||
|
tlr2 (rat) [NM_198769] |
Forward | CTCCTGTGAACTCCTGTCCTT | 74 | 60 |
| Reverse | AGCTGTCTGGCCAGTCAAC | |||
|
tlr3 (rat) [NM_198791] |
Forward | GATTGGCAAGTTATTCGTC | 205 | 54 |
| Reverse | GCGGAGGCTGTTGTAGG | |||
|
tlr4 (rat) [NM_019178] |
Forward | GATTGCTCAGACATGGCAGTTTC | 135 | 54 |
| Reverse | CACTCGAGGTAGGTGTTTCTGCTAA | |||
|
tlr5 (rat) [NM_001145828] |
Forward | GGGCAGCAGAAAGACGGTAT | 61 | 60 |
| Reverse | CAGGCACCAGCCATCCTTAA | |||
|
tlr6 (rat) [NM_207604] |
Forward | AGAACCTTACTCATGTCCCAAAAGAC | 79 | 60 |
| Reverse | AGATCAGATATGGAGTTTTGAGACAGACT | |||
|
tlr7 (rat) [NM_001097582] |
Forward | GTTTTACGTCTACACAGTAACTCTCTTCA | 75 | 60 |
| Reverse | TTCCTGGAGGTTGCTCATGTTTT | |||
|
tlr8 (rat) [NM_001101009] |
Forward | GGGGTAACACACCGTCTA | 150 | 60 |
| Reverse | GTCAAGGCGATTTCCACT | |||
|
tlr9 (rat) [NM_198131] |
Forward | CCGAAGACCTAGCCAACCT | 70 | 60 |
| Reverse | TGATCACAGCGACGGCAATT | |||
|
ifn-β (rat) [NM_019127] |
Forward | CTTGGGTGACATCCACGACTAC | 92 | 54 |
| Reverse | GGCATAGCTGTTGTACTTCTTGTCTT | |||
|
il-6 (rat) [NM_012589] |
Forward | AAGAAAGACAAAGCCAGAGTC | 263 | 60 |
| Reverse | CACAAACTGATATGCTTAGGC | |||
|
mmp3(rat) [NM_133523] |
Forward | ATCCCCTGATGTCCTCG | 147 | 54 |
| Reverse | TTTCGCCAAAAGTGCC | |||
|
mmp13 (rat) [NM_133530] |
Forward | TTCAACCCTGTTTACCT | 293 | 54 |
| Reverse | TTCTTTTTCCTTGTCCC | |||
|
β-actin (rat) [NM_031144] |
Forward | GAGGGAAATCGTGCGTGAC | 157 | 60 |
| Reverse | GCATCGGAACCGCTCATT | |||
|
TLR3 (human) [NM_003265] |
Forward | AGCCTTCAACGACTGATGCT | 201 | 60 |
| Reverse | TTTCCAGAGCCGTGCTAAGT | |||
|
β- ACTIN (human) [NM_001101] |
Forward | AGTTGCGTTACACCCTTTCTTG | 150 | 60 |
| Reverse | TCACCTTCACCGTTCCAGTTT | |||