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. 2011 Jun 10;13(3):R59. doi: 10.1186/bcr2896

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Copyright ©2011 Kallergi et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Triple-immunofluorescence (CK/Twist/CD45, CK/vimentin/CD45) in CTCs. Representative ARIOL system photomicrographs of CTC cytospin after negative immunomagnetic separation in patients with metastatic breast cancer. (A) Cells were triple-stained with pan-CK A45-B/B3 antibody/Zenon Alexa Fluor 488 (green)/Twist anti-rabbit antibody/Alexa Fluor 555 anti-rabbit antibody (orange) and CD45 anti-mouse/Alexa Fluor 633 anti-mouse antibody (blue). Original magnification, x400. (B) Cells were triple-stained with pan-CK A45-B/B3 antibody/Zenon-Alexa Fluor 488 (green)/vimentin anti-rabbit antibody/Alexa Fluor 555 anti-rabbit antibody (orange) and CD45 anti-mouse/Alexa Fluor 633 anti-mouse antibody (blue). Original magnification, x400. ARIOL system = automated image analysis system; CK = cytokeratin; CTCs = circulating tumour cells.

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