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. 2011 Jun 29;13(3):R70. doi: 10.1186/bcr2913

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Copyright ©2011 Bergamaschi et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Regulation of 14-3-3 family members in MCF-7 breast cancer cells upon ligand treatment. (a) Expression of 14-3-3 isoforms at the indicated times (0, 4, 24, 48 hours) after 1 μM tamoxifen (Tam) treatment. 14-3-3σ is expressed at a very low level and is therefore not shown. Inset shows 14-3-3ζ protein evaluated by western blot after Tam treatment for 0 to 72 hours with β-actin as the loading control. (b) Cells were cultured in the presence of vehicle (0.1% ethanol), 1 μM Tam, 10 nM E2, 1 μM ICI 182,780 (ICI) alone or ICI in combination with 10 nM Tam for various times. RNAs were measured by real-time PCR..