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. 2011 Jun 29;13(3):R70. doi: 10.1186/bcr2913

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Copyright ©2011 Bergamaschi et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Characterization of the phenotypic properties of MCF-7 cells with knockdown of 14-3-3ζ. (a) 14-3-3ζ RNA and protein levels were evaluated by quantitative PCR and western blot in cells stably expressing control shRNA (Ctrl) or 14-3-3ζ shRNA knockdown (KD) and in KD cells transfected with wild type 14-3-3ζ (KD_R, knockdown and reexpression). (b) Cell viability in response to different concentrations of tamoxifen (Tam) for 48 hours for Ctrl or 14-3-3ζ KD or KD_R cells. Cell number for vehicle-treated control cells is set as 100%. (c) Percentage of cells in the different cell cycle stages for Ctrl and 14-3-3ζ KD or KD_R cells treated with 1 μM Tam for 72 hours. (d) Percentage of apoptotic cells in Ctrl, 14-3-3ζ KD, and KD_R cells treated with 1 μM Tam.