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A, Double labeling for kisspeptin (red) and LHRH (green) indicates that the LHRH neurons do not coexpress kisspeptin. Bar, 25 μm. B, In the ME (external zone), in which the LHRH neurons terminate, although numerous kisspeptin axons are found (red), those kisspeptin terminals are distinct from the LHRH terminals (green), supporting the notion that the LHRH neurons do not coexpress kisspeptin. Bar, 25 μm.
