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. 2010 Nov 24;152(1):138–150. doi: 10.1210/en.2010-0791

Figure 3.

Figure 3

The GC-mediated transcriptional changes in MIN6 cells are GR-mediated. MIN6 cells were stimulated for 12 h with the GR-selective agonist dexamethasone, the MR-selective agonist aldosterone, or the general GR/MR agonist hydrocortisone alone or in combination with RU486 (GR antagonist) or spironolactone (MR antagonist) as indicated. Dexamethasone and hydrocortisone robustly inhibit the expression of CRFR1 (A) and GLP-1R (B), which is negated by co-administration of RU486, but not spironolactone, both at equimolar levels. Expression of neither gene is affected by aldosterone. In contrast, the expression of CRFR2, as detected by generic (C) or CRFR2α-specific (D) primers, is potently increased by dexamethasone and hydrocortisone. These increases are partially prevented by RU486, but not spironolactone, indicating that these changes are GR-dependent. The expression of GIPR (E) and Ucn 3 (F) is inhibited in a pattern that reminisces the expression of CRFR1 and GLP-1R, although this inhibition is less robust. Data are normalized to HPRT expression and expressed relative to untreated controls. Error bars indicate the SE of quadruplicate treatments, asterisks indicate statistically significant differences with controls unless indicated otherwise (*, P < 0.05; **, P < 0.01; ***, P < 0.001).