Figure 4.

Hsp27 association with eIF4E involves its C-terminal region. (a) Schematic representation of Hsp27 structure and deletion mutants used in this study. (b) Western blot analysis of histidine and eIF4E protein levels after eIF4E immunoprecipitation (IP) using rabbit anti-eIF4E antibody (reIF4E) or rabbit IgG as control in MiaPaCa-2 cells transiently transfected with WT Hsp27 or truncated mutant forms (N1, N2 and C1) of Hsp27. In the lower panel, western blot analysis of histidine and vinculin levels from total protein extracts (TCL). (c) MTT quantification of cell viability of MiaPaCa-2 cells transiently transfected or not with Hsp27 deletion mutants and treated with gemcitabine (150 mM). Error bars, S.E. from three independent experiments. Statistical analysis used t test; ^***P≤0.001