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. 2011 Oct 20;2(10):e221. doi: 10.1038/cddis.2011.104

Figure 5.

Figure 5

Hsp27-eIF4E interaction depends on Hsp27 phosphorylation. (a) Schematic representation of WT and phosphorylation mutants (3D and 3A) of Hsp27 used in this study. (b) Western blot analysis of histidine and eIF4E protein levels after eIF4E immunoprecipitation (IP) using rabbit anti-eIF4E antibody (reIF4E) in MiaPaCa-2 cells transiently transfected with Hsp27 WT or 3D (constitutively phosphorylated) and 3A (constitutively dephosphorylated) vectors. In the lower panel, western blot analysis of histidine and vinculin levels from total protein extracts (TCL)