Figure 4.

Reduced activation of ERK and p38 upon c-FLIP downregulation. HeLa-CD95, HeLa-CD95-F ctrl, and HeLa-CD95-F dr cells were stimulated with 1000 ng/ml anti-APO-1 for the indicated time periods. Total cellular lysates were analyzed by SDS-PAGE and IB using the indicated primary antibodies. Representative IB results for ERK (a) and p38 (c) activation as well as the averaged quantified signals of scaled time courses with standard deviations of all performed experiments (b and d) are shown. Displayed results are representative for four independent experiments. HeLa-CD95-F dr, HeLa-CD95 cells with c-FLIP downregulation; HeLa-CD95-F ctrl, control cell line without c-FLIP downregulation. Independent repeats of these experiments are shown in Supplementary Figure S4