Figure 7.

Altered ratio of c-FLIP/C8 at the DISC upon caspase-8 downregulation and altered kinetics with lower stimulus at the DISC. HeLa-CD95 cells and HeLa-CD95-C8 dr cells were treated with 1000 or 250 ng/ml anti-APO-1 for the indicated time periods and IP of the DISC was performed. Total cellular lysates and IPs of the DISC were analyzed by SDS-PAGE and IB using the indicated primary antibodies. Representative IB results (a and c) and the quantification for the displayed IB (b and d) are shown. Displayed results are representative of two independent experiments. HeLa-CD95-C8 dr, HeLa-CD95 cells with caspase-8 downregulation. Asterisks indicate nonspecific bands. Independent repeats of these experiments are shown in Supplementary Figure S10