Figure 4.

TEM of 3D7 parasites. (ai and ii) Untreated 3D7 parasites show clearly defined hemazoin crystals located within an intact DV membrane. (bi and ii) Parasites exposed to 3 μM of CQ for 4 h also show intact DV membrane but with less discrete hemazoin crystals and membrane whorls forming inside the DV. (ci–iii) Some of the treated parasites also show compromised DV membrane with DV material extruding into the cytoplasm. (d) Other CQ-treated parasites show DV rupture leading to hemazoin crystals being dispersed into the cytoplasm. Scale bar shows 0.5 μm for × 11 000 and × 18 000 magnifications, 0.2 μm for × 28 000 and × 36 000 magnifications, and 0.1 μm for × 56 000 magnification