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. 2011 Oct 31;108(46):18766–18771. doi: 10.1073/pnas.1116273108

Fig. 3.

Fig. 3.

IRF2 binds to the promoter region of trypsinogen5 gene. (A) The effects of siRNAs (3 μg) specific to IRF2 or a control scrambled sequence on transcriptional activity of the −216 to +15 luciferase reporter in TGP49 acinar cells were measured. *P < 0.05 versus control siRNA. (B) A chromatin immunoprecipitation assay was done using TGP49 acinar cells with the IRF2-specific antibody (5 μg) or the same amount of control nonspecific IgG. The precipitated chromatin fragments were detected by PCR with a trypsinogen5 promoter-specific primer set at 35 cycles or a negative control primer set for angiotensinogen (Agt) exon2 at 30 (Upper) and 35 (Lower) cycles. The input before precipitation indicates the predicted size (Trp5, 229 bp; Agt, 221 bp) of the PCR product. (C) The ChIP assay done in B was quantitatively measured using a real-time PCR method with the same primers. The relative amounts of β-actin were calculated, and the amounts of chromatin fragments precipitated with the anti-IRF2 antibody were shown relative to those with the nonspecific control antibody (IgG). *P < 0.01 versus control IgG.