Fig. 3.
Insulin signaling regulates enterocyte differentiation. (A) dInRE19 mutant, dInR339 mutant, and control clones 7 d ACI from females reared on a rich diet. Clone, GFP green; Delta (yellow vesicular); Prospero (yellow nuclear); Pdm1 (red, nuclear) and DAPI (blue, nuclear). Asterisks mark mature enterocytes (Pdm1-positive cells). Dotted lines outline clone boundaries. (B) A clone overexpressing dInRA1325D in all cells contains a large enterocyte (yellow asterisks), an ISC (arrowhead), and an enteroendocrine cell (arrow). Clone, GFP green; Delta, red vesicular (Inset and merge); Prospero, red nuclear (Inset and merge); DAPI, blue nuclear. Arrowheads and arrows indicate, respectively, ISCs (Delta-positive) and enteroendocrine (Prospero-positive) cells in A and B. (Scale bar, 20 μm in A and B.) (C) Wild-type ISC clones contain more cells than ISC clones overexpressing an activated form of the insulin receptor (dInRA1325D).