. 2011 Nov 2;108(46):18678-18683. doi: 10.1073/pnas.1113813108

Table 1.

Biophysical properties of PGC1α220/ERRγLBD binary complex and its components

	SEC^*	MALS^†		SV		Siegel and Monty	QELS^†
Protein	R_s (Å)	Molar Mass^‡	Stochiometry^§	S_20,w	f/f_o	Molar Mass^‡	R_h (Å)
PGC1α220	42.9 ± 0.5	23,945 ± 3,434	Monomer (24,456)	1.3	2.0	22,598	ND^¶
ERRγLBD	33.2 ± 0.13	53,673 ± 3,094	Dimer (52,928)	3.8	1.2	51,119	25.7 ± 1.2
Complex	51.7 ± 1.8	74,175 ± 473	1∶2 (77,386)	4.0	1.5	82,747	ND

^*The results presented are the average of 2–4 replicates.

^†SEC-MALS-quasi-elastic light scattering (QELS) measurements were made at room temperature.

^‡Molar masses are presented in units of daltons.

^§Stoichiometry as inferred from experimentally determined molecular mass. In parentheses, theoretical values as computed from primary sequence using SEDNTERP are presented.

^¶ND, not determined