Table 2.

Characterization of secondary structure and thermal and chemical stability of the complex by CD and fluorescence

		PGC1α220	ERRγLBD	Binary complex
Secondary structure*	% alpha	21.69 (47-aa)	68.06 (157-aa)	52.38 (236-aa)
	% beta	5.39	2.35	4.76
	% coil	72.91	29.59	42.86
Thermal denaturation	Tm† [K]	Indeterminate	324.42 ± 0.059750	328.51 ± 0.055400
Chemical denaturation	Dm† [M]urea	Indeterminate	3.25 ± 0.095	3.47 ± 0.075

^*Secondary structure estimation was calculated using CDFIT.

^†T_m and D_m values reported for each species are obtained from an average of three different experiments. The error reported is the standard deviation.