Fig. 3.

Galectin-12 deficiency results in elevated lipolysis in adipocytes. (A and B) Adipocytes were isolated from epididymal fat depots of Lgals12^+/+ and Lgals12^−/− mice (n = 3–6) on a regular diet ad libitum. Equal numbers of cells were incubated in the absence (A) or presence (B) of 0.1 μM of isoproterenol. Glycerol and nonesterified fatty acid (NEFA) released into the medium were measured at different time points of incubation at 37 °C. (C) siRNA-mediated knockdown was performed by electroporating 3T3-L1 adipocytes with either control siRNA (Ctrl) or a combination of two galectin-12–specific siRNAs (si12). Three days later, galectin-12 levels were analyzed by Western blotting, and lipolysis was determined by monitoring the release of fatty acids and glycerol 1.5 h after isopreterenol stimulation. Asterisks denote statistical significance (*P < 0.05). Similar results were observed in four (A and B) or three (C) experiments.