Fig. 4.
Tissue lipid content, total ambulatory activity, energy expenditure, and food intake in Lgals12+/+ and Lgals12−/− mice. (A) Lipid contents of liver and muscle were determined for Lgals12+/+ and Lgals12−/− male mice (n = 5 for each genotype), by the ethanolic-KOH saponification method. (B) Food intake of Lgals12+/+ (n = 5) and Lgals12−/− (n = 6). (C and D) Total ambulatory activity (distance of movement) and oxygen consumption rates (VO2) were determined by indirect calorimetry during the dark (7:00 PM to 7:00 AM) and light (7:00 AM to 7:00 PM) periods in Lgals12+/+ and Lgals12−/− male mice (n = 5 for each genotype) on standard diet. (E and F) Basal (E) or isoproterenol-stimulated (F) oxygen consumption of isolated epididymal white adipocytes from Lgals12+/+ and Lgals12−/− mice (n = 5–7) measured with the BD Oxygen Biosensor System. Oxygen consumption in E is expressed as normalized relative fluorescence unit (NRFU). Results in F are the ratios of oxygen consumption by adipocytes stimulated with isoproterenol to that by adipocytes under basal conditions. All animals were studied at 22 to 26 wk of age. Asterisks denote statistical significance (*P < 0.05).