Fig. 5.

Transmembrane view of disulfide-locked Rosetta Membrane structural models of the voltage-sensing domain of NaChBac. Segments S1 through S4 colored individually and labeled. Side chains of cysteine mutants of E43, T0, R1, R2, and R3 are shown in space-filling representation and colored yellow. Cβ atoms of gating-charge-carrying arginines (blue; labeled R1 through R4) in S4 and D60 and E70 (red) in S2 shown as spheres. Models were generated with the Rosetta Membrane modeling system (SI Methods). This figure was generated using Chimera (43).