Fig. 2.

Prevalence of P. falciparum pyrimethamine- and cycloguanil-resistant DHFR mutants in 796 malaria vector mosquitoes. Spray catches were obtained. Midguts (A) and salivary gland (B) specimens were separated, and DNA was PCR amplified and analyzed for allele-specific polymorphisms by restriction digestion confirming midgut infections (n = 81) and mosquito gland infections (n = 62). y-axis numerals denote DHFR codons. Represented are WT, pyrimethamine-resistant mutants (S108N, N51I, C59R, and triple mutants), cycloguanil-resistant mutants (S108T, A16V, and double mutant), and mixed alleles (WT+S108T or designated mutant+S108T).