Table 1.

Determinations of P. falciparum DHFR resistant polymorphisms for parasites from blood smear-positive and -negative humans and from vector mosquito midguts and salivary glands

Infection	S108N	N51I	C59R	S108N+N51I+C59R	A16V	I164L	108T+16V
Human blood smear-positive
Mut	67	60	37	36	0	0	0
S108T	0	0	0	N/A	0	0	0
Mut + S108T	0	0	0	N/A	0	0	0
WT + mut	11	6	11	N/A	0	0	0
WT	6	15	34	43	84	74	84
Total	84	81	82	79	84	74	84
Human blood smear-negative
Mut	43	33	29	10	2	0	2
S108T	5	0	0	N/A	0	0	0
Mut + S108T	2	0	0	N/A	0	0	0
WT + mut	0	0	0	N/A	0	0	0
WT	2	11	18	22	44	39	44
Total	52	44	47	32	46	39	46
Mosquito midgut
Mut	7	6	1	1	38	1*	33
S108T	71	0	0	N/A	0	0	0
Mut + S108T	2	0	0	N/A	0	0	0
WT + mut	0	0	0	N/A	0	1	0
WT	1	43	62	40	29	44	34
Total	81	49	63	41	67	46	67
Mosquito salivary gland
Mut	27	7	11	4	2	0	1
S108T	26	0	0	N/A	0	0	0
Mut + S108T	0	0	0	N/A	0	0	0
WT + mut	1	0	0	N/A	0	0	0
WT	8	46	43	46	40	42	41
Total	62	53	54	50	42	42	42

Human blood smear-positive, n = 86; human blood smear-negative, n = 88; mosquito midgut, n = 81; mosquito salivary gland, n = 64. N/A, not applicable.

*Midgut, I164R.