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. 2011 Nov 2;108(46):18831–18836. doi: 10.1073/pnas.1114569108

Fig. 1.

Fig. 1.

Characterization of the Ddysb mutant. (A) Transposon insertion site. (B) Representative Western blots and group data showing reduced expression of Ddysb in the head of the dysb1 mutant (t test; P = 0.007; n = 5). (C) The dysb1 mutant shows decreased EJC amplitude (t test; P = 0.002, 0.02, 0.03, and 2.1E-6 for calcium concentrations of 0.15, 0.2, 0.3, and 0.4 mM, respectively; n = 7–14). (D) Dopamine concentration in whole-head extracts of adult flies is elevated significantly in the dysb1 mutant (t test; P = 1.9E-6; n = 8–10). (E) The dysb1 mutant shows memory defects in the Pavlovian olfactory aversive conditioning (t test; P = 0.004, 0.002, and 0.009; n = 7–8). The T-maze for memory test is illustrated. (F) dysb1 mutants show dramatically increased locomotor activity (t test; P = 2E-4; n = 17). The experimental paradigm for the locomotion test is illustrated on the left. (G) The dysb1 mutant shows mating disorientations (t test; P = 0.02; n = 28–32). The mating preference assay is illustrated. Data are means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.