Fig. 3.
TR2 and TR4 bind to the fetal γ-globin gene promoter in spleen cells of SCD:TgTR2/TR4 mice. Binding of TR2 and TR4 to the human fetal γ-globin gene proximal promoter region containing the DR sequence, as well as the adult β-globin gene promoter in spleen cells of SCD or SCD:TgTR2/TR4 mice, was analyzed by ChIP assay using anti-TR2 or -TR4 antibody, or control IgG. For a negative control (Cont), an intronic region of the murine CHMP4B gene was also analyzed. Two mice of each genotype were used for the analysis. Error bars represent SD.